132-32-13-氨基-9-乙基咔唑

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商品编号：A105545

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商品名称：132-32-13-氨基-9-乙基咔唑
商品毛重：0.00
单位：件
上架时间：2017-10-31 22:35:48

名称: 3-氨基-9-乙基咔唑英文名称: 3-Amino-9-ethylcarbazole95%产品编号 A105545
CAS号: 132-32-1分子式 C₁₄H₁₄N₂分子量210.28
MDL号 MFCD00004964

标识符号	GHS06, GHS08
信号词	Danger
风险声明 (欧洲)	R25;R36/37/38;R40
安全声明（欧洲）	S26;S36/37/39
警示性声明	P201,P301+P310,P308+P313
危害码（欧洲）	T
危害声明	H301,H350
个人防护设备	Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

属性

密度	1.1700
熔点	98°C-100°C
存贮条件	储存温度 2-8°C

描述

产品介绍	AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .
别名	AEC, 50X ;3-氨基-N-乙基咔唑;AEC, 50X
生化机理	3-氨基-9-乙基咔唑是一种过氧化酶底物，适用于免疫印迹和免疫组织化学染色程序。该底物能产生一种不溶性红色的末端产物。肉眼或可见光下就可观察。
应用	过氧化物酶底物. Usage ? Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps. ? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;). ? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well. ? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT. ? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain. ? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; ) ? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations